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Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes

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dc.contributor.author Tahir, Ahmed EL
dc.contributor.author Malhotra, Pawan
dc.contributor.author Chauhan, Virander S
dc.date.accessioned 2018-07-22T06:31:19Z
dc.date.available 2018-07-22T06:31:19Z
dc.date.issued 2003-05-07
dc.identifier.uri http://repo.uofg.edu.sd/handle/123456789/836
dc.description Published: 7 May 2003 Malaria Journal2003, 2:11 Received: 3 February 2003 Accepted: 7 May 2003 This article is available from: http://www.malariajournal.com/content/2/1/11 © 2003 Tahir et al; licensee BioMed Central Ltd. This is an OpenAccess article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL en_US
dc.description.abstract Background: Intraerythrocytic malaria parasites actively import obligate nutrients from serum and export proteins and lipids to erythrocyte cytoplasm and membrane. The import of macromolecules in the malaria parasite has been the subject of many debates. To understand the import of macromolecules by the parasite, we studied the uptake of proteins by Plasmodium falciparum infected human erythrocyte. Methods: Proteins were biotin labelled individually, purified on a gel filtration column and added to uninfected and infected asynchronized culture. The uptake of these proteins by malaria parasites was determined by western blot analysis of parasite pellet and their different fractions using streptavidin-horseradish conjugate. To further confirm this import, we studied the uptake of 125 Ilabelled proteins by western blot analysis as well as used direct immunofluorescence method. Results: Here we show that biotin labelled and radio-iodinated polypeptidesof molecular sizes in the range of 45 to 206 kDa, when added in the culture medium, get direct access to the parasite membrane through a membrane network by by-passing the erythrocyte cytosol. The import of these polypeptides is ATP-dependent as sodium azide treatment blocks this uptake. We also show that malaria parasites have the ability to take up and degrade biotin labelled human serum albumin, which has been shown to be essential for the parasite growth. Conclusions: These results can be used, as a basis toexplore the role of human serum albumin in the intraerythrocytic development of parasites, and this in turncan be an important adjunct to the development of novel antimalarial drugs. en_US
dc.language.iso en en_US
dc.publisher Malaria Journal en_US
dc.subject malaria parasites en_US
dc.subject malaria en_US
dc.title Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes en_US
dc.type Article en_US


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