Biological Analysis of Exon 5 in P53 Gene among Non-Hodgkin Lymphoma Patient's using DNA Sequencing Technique, National Cancer Institute, Gezira State, Sudan (2017)

Abstract

Non-Hodgkin lymphoma (NHL) is the diverse group of blood cancers that share a single characteristic. Generally develops in the lymph nodes or in the lymphatic tissue found in organs, which arises from an injury to the DNA of a lymphocyte. The altered DNA in one lymphocyte produces a malignant transformation. Inactivation of the tumor suppressor gene (TP53) by various genetic alterations is a major event in human tumorgenesis. Epidemiological studies of the hematological malignancies indicate that the prognosis of patients with a mutation in the p53 gene is inconsistent. The aim of this study was to detect mutations in p53 (exon5) gene in Sudanese NHL patients. This is a cross-section study was conducted in National Cancer Institute in Gezira state from April- September 2017. Thirty patients (17 males and 13 females) were examined which include different subtypes (4 Burkitt's lymphoma, 16 Diffuse large cell lymphoma,2 Follicular lymphoma, 3 lymphoblastic lymphoma, 1 Mantle cell lymphoma, 1 marginal zone lymphoma, 1 mixed small & large lymphoma and 2 small lymphocytic lymphoma). The age group from 4-70 years old with mean (37 years). The demographic data of each patient and control were collected using questionnaire. 2-3 ml of blood was collected in EDTA container for DNA extraction, the purified DNA was introduced for amplification by using polymerase chain reaction(PCR), in order to replicate the P53gene (exon5), then the products about 252 bp in size were evaluated by gel electrophoresis. The products of genomic DNA were sent for sequencing to detect mutations using Sanger technique. The results were analyzed by using Finch TV software and Bioedit to compare the multiple sequence alignment of the patient's and control's sequence to find the differences, also s no differences were found when used the BLAST software. There was no mutation detected in p53 (exon5) in NHL Sudanese patients. The use of other bioinformatics tools is recommended.