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Assessment of Molecular Diagnosis Methods for Abdominal Tuberculosis and Tuberculous Lymphadenitis among Patients in Gezira State, Sudan

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dc.contributor.author Al jack, Moayad Abdulslam El ha
dc.date.accessioned 2018-10-22T09:48:53Z
dc.date.available 2018-10-22T09:48:53Z
dc.date.issued 2014-03-29
dc.identifier.uri http://repo.uofg.edu.sd/handle/123456789/2515
dc.description A Thesis Submitted to the University of Gezira in Fulfilment of the Requirements for the award of the Degree of Master of Science (M.Sc) in MOLECULAR BIOLOGY Department of Molecular Biology NATIONAL CANCER INSTITU March 2014 en_US
dc.description.abstract Extrapulmonary tuberculosis is considered an important clinical problem. The aim of this study was assess two molecular methods kit (CinnaGen Inc Iran) and IS6110 gene and two different samples (blood and fluid) for the diagnosis of abdominal tuberculosis and tuberculous lymphadenitis. The study subjects were recruited from Wad Medani teaching hospital during 2009-2013. Seventy five ascitic fluid and blood samples were collected from each patients suspected to have abdominal tuberculosis and twenty five lymph aspirate and blood were collected from each suspected tuberculous lymphadenitis. DNA was extracted using DNPTM kit (CinnaGen Inc) and polymerase chain reaction (PCR) was done using a kit (CinnaGen Inc) and IS6110 gene for both samples. In abdominal tuberculosis, the comparison between ascitic fluid and blood samples when kit were used, 10/75 (13%) and 3/75 (4%) were positive respectively, when IS6110 gene used, 20/75 (27%) and 9/75 (12%) were positive respectively, there was a significant difference in both results, P-value < 0.05. When the two diagnostic methods, kit and IS6110 gene were compared with each other, 10/75 (13%) and 20/75 (27%) were positive respectively, a significance difference was found, in case of ascitic fluid samples, P-value < 0.05, there was no significance in case of blood samples (3/75 (4%) 9/75 (12%) respectively ), P- Value > 0.05, The correlation between the clinical presentation of the study subjects and PCR using IS6110 gene, showed that 12/75 (16%) were clinically highly suggestive while 20/75 (27%) were positive a significant difference was found, P- Value < 0.05. In tuberculous lymphadenitis, the comparison between aspirate and blood samples when kit and IS6110 gene were used, 7/25 (28%) and 1/25 (4%), 13/25 (52%) and 3/25 (12%) were positive respectively, with a significant difference P- Value < 0.05. The comparison between the two methods CinnaGen kit and IS6110 gene, 7/25 (28%) and 13/25 (52%) were positive respectively, in case of aspirate. while in case of blood 3/25 (12%) and 1/4 (4%) were positive respectively, there was no significant difference, P-Value > 0.05 in both cases. The correlation between the clinical presentation of the study subjects and PCR using IS6110 gene, showed that 13/25 (52%) of the tuberculous lymphadenitis cases were clinically highly suggestive, while 10/25 (40%) were positive with a significant difference P- Value < 0.05. This study conclude that the best sample for diagnosis of abdominal TB and Lymphadenitis is ascitic fluid and LN aspirate and the best diagnostic method to use is IS6110 gene. Clinical presentation is important to identify extrapulmonary tuberculosis cases. In this study, ascitic fluid and lymph aspirate samples is recommended to be used in molecular diagnostic test. IS6110 insertion sequence gene is the best method to be used. Clinician should put more emphasis in the on clinical criteria for diagnosis extrapulmonary tuberculosis. en_US
dc.description.sponsorship . Nagla Gasmelseed Mohammed Ahmmed ( Main Supervisor) . Osman Khalfala Saeed Taha (Co-supervisor) . Elgaili Mohamed Elgaili Al ameen ( Co-supervisor) en_US
dc.language.iso en en_US
dc.publisher University of Gezira en_US
dc.subject Molecular diagnostics en_US
dc.subject Tuberculosis outside the lung en_US
dc.subject Abdominal diseases en_US
dc.title Assessment of Molecular Diagnosis Methods for Abdominal Tuberculosis and Tuberculous Lymphadenitis among Patients in Gezira State, Sudan en_US
dc.type Thesis en_US


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