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Performance of Immunofluorescence on Paraffin Wax Embedded Sections in The Diagnosis of Lupus Nephritis Compared to Immunofluorescence on Frozen Sections (2011-2013)

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dc.contributor.author Ismail, Omer Hassan Omer
dc.date.accessioned 2018-09-23T11:35:57Z
dc.date.available 2018-09-23T11:35:57Z
dc.date.issued 2014-04-16
dc.identifier.uri http://repo.uofg.edu.sd/handle/123456789/1909
dc.description A Thesis Submitted to the University of Gezira in Fulfillment of the Requirements for the Award of the Degree of Master of Medical Laboratory Sciences in Histopathology Department of Histopathology and Cytology ,Faculty of Medical Laboratory Sciences April, 2014 en_US
dc.description.abstract Systemic lupus erythematosus (SLE), is a chronic inflammatory autoimmune disease with multisystem involvements, it often harms the heart, joints, skin, lung, blood vessels, liver, kidneys and nervous system. The laboratory diagnosis depends on the routine stain (Haematoxylin and Eosin), and ancillary studies such as immunofluorescence, immunohistochemistry and electron microscopy. The disease may occur at any age, but the most common between the age of 15 and 40 years with a female preponderance of 4 to 1. There is no one specific cause of SLE, there are, however, a number of environmental triggers and a number of genetic suscepilities. The accurate diagnosis of SLE is based on a combination of clinical finding and laboratory evidence. The objective of the present study was to apply the Immunofluorescence technique on paraffin sections to help in diagnosis of SLE in Sudan, because the Immunofluorescence technique on frozen and paraffin sections for immunoglobulins and complement is often the primary approach for the differential diagnosis of glomerular diseases. We carried out the study between March 2011 to April 2013, on 64 patient with lupus nephritis , and the Immunofluorescence technique on frozen and paraffin sections were used to study the renal biopsies. A computer program was used for data analysis. Fifty four patients were females (84.4%) and ten were males (15.6%). This study revealed that the sensitivity of Immunofluorescence on frozen sections (81.3%) was slightly higher than Immunofluorescence on paraffin sections (70.3%). Conclusively, frozen section technique can be effectively replaced by paraffin wax technique, if some modifications are introduced to reach the optimum time in order to achieve a high sensitivity. en_US
dc.description.sponsorship Ali Sid Ahmed Mohammed (Main Supervisor) Shams Eldeen Mohammed Ahmed (Co-Supervisor) en_US
dc.language.iso en en_US
dc.publisher University of Gezira en_US
dc.subject Systemic lupus erythematosus en_US
dc.subject autoimmune disease en_US
dc.subject harms the heart en_US
dc.subject blood vessels en_US
dc.subject joints en_US
dc.subject nervous system en_US
dc.subject genetic suscepilities en_US
dc.title Performance of Immunofluorescence on Paraffin Wax Embedded Sections in The Diagnosis of Lupus Nephritis Compared to Immunofluorescence on Frozen Sections (2011-2013) en_US
dc.type Thesis en_US


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