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Expression of the Isochrysis C18-D9 Polyunsaturated Fatty Acid Specific Elongase Component Alters Arabidopsis Glycerolipid Profiles1

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dc.contributor.author Fraser, Thomas Colin Michael
dc.contributor.author Qi, Baoxiu
dc.contributor.author Elhussein, Salah
dc.contributor.author Chatrattanakunchai, Sunantha
dc.contributor.author Stobart, Allan Keith
dc.contributor.author l Lazarus, Colin Michae
dc.date.accessioned 2018-07-31T11:31:30Z
dc.date.available 2018-07-31T11:31:30Z
dc.date.issued 2004
dc.identifier.uri http://repo.uofg.edu.sd/handle/123456789/1038
dc.description Plant Physiology, June 2004, Vol. 135, pp. 859–866, en_US
dc.description.abstract A cDNA isolated from the prymnesiophyte micro-alga Isochrysis galbana, designated IgASE1, encodes a fatty acid elongating component that is specific for linoleic acid (C18:2n-6) and a-linolenic acid (C18:3n-3). Constitutive expression of IgASE1 in Arabidopsis resulted in the accumulation of eicosadienoic acid (EDA; C20:2n-6) and eicosatrienoic acid (ETrA; C20:3n-3) in all tissues examined, with no visible effects on plant morphology. Positional analysis of the various lipid classes indicated that these novel fatty acids were largely excluded from the sn-2 position of chloroplast galactolipids and seed triacylglycerol, whereas they were enriched in the same position in phosphatidylcholine. EDA and ETrA are precursors of arachidonic acid (C20:4n-6), eicosapentaenoic acid (C20:5n-3), and docosahexaenoic acid (C22:6n-3) synthesized via the so-called v6 D8 desaturase and v3 D8 desaturase biosynthetic pathways, respectively. The synthesis of significant quantities of EDA and ETrA in a higher plant is therefore a key step in the production of very long chain polyunsaturated fatty acid in oil-seed species. The results are further discussed in terms of prokaryotic and eukaryotic pathways of lipid synthesis in plants. en_US
dc.language.iso en en_US
dc.publisher Plant Physiology, June 2004, Vol. 135, pp. 859–866, www.plantphysiol.org en_US
dc.subject Polyunsaturated en_US
dc.subject Fatty Acid en_US
dc.subject tissues examined en_US
dc.subject plant morphology en_US
dc.subject phosphatidylcholine en_US
dc.subject lipid synthesis in plants. en_US
dc.title Expression of the Isochrysis C18-D9 Polyunsaturated Fatty Acid Specific Elongase Component Alters Arabidopsis Glycerolipid Profiles1 en_US
dc.title.alternative Plant Physiology, June 2004, Vol. 135, pp. 859–866, www.plantphysiol.org en_US
dc.type Article en_US


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